Laboratory for Diagnosis of Bacterial and Viral Coinfections

Major areas of research:

The study of character of the pathological process at the sub-cellular level with the co-infection of the urogenital tract, using molecular genetic techniques, ultrastructural, nanoscopic analysis.
Improving of the methods of indication and identification of viruses and bacteria in renal, reproductive, osteo-articular pathology.
Identification of virulence factors, peculiarities of morphogenesis, molecular-genetic criteria, including sensitivity to chemotherapy drugs strains of C. trachomatis, T. vaginalis, M. genitalium et al., circulating in the Republic of Belarus.
Development of nanodiagnostics methods, the creation of a test system using a functionally-active nanoparticles, and highly sensitive sensor coatings for detecting of C. trachomatis persistent form infections.
Develop of criteria intravital laboratory diagnosis of neurodegenerative diseases, including Alzheimer's type dementia and prion infection.

Scientific projects:

1. Develop technology microbiological and molecular genetic diagnosis and treatment etiopathogenetic necrosis of the femoral head in the early stages of its development in young patients.

2. To identify predictors of the development of neurodegenerative process Alzheimer's disease in order to improve differential diagnosis and treatment.

3. Conduct molecular epidemiological monitoring of Chlamydia trachomatis genotypes, circulating on the territory of the Republic of Belarus, to study their virulence and resistance to antibiotics.

Lab Equipment:

Electron microscope JEM-1011 (Japan)
Light microscope Nicon (Japan)
Atomic Force Microscope Nano-scope 3D Multimode (Veeco, USA)
Immunoassay Analyzer automatically Evolis Twin Plus (Bio-Rad, France)
Thermal Cycler Gradient Palm-Cycler (Corbett Research Pty Limited, Australia)
Labculture Class II Type B2 (Total Exhaust) Biosafety Cabinet (E-Series) (ESCO, USA)
Biosafety Cabinet KOJAIR KR-125 (Finland)
Centrifuge MPW-350R (Poland)

Main methods:

Enzyme-linked immunosorbent assay (ELISA)
PCR
Immunofluorescence
Cultivation of Chlamydia trachomatis in McСoy cell cultures
Electron microscopy
Atomic Force Microscopy

Main Publications:

Gavrusev А., Strockij А., Rubanik L., Poleshhuk N. Sensitivity of Chlamydia trachomatis to antibiotics and Trichomonas vaginalis to antiprotozoal drugs in men with chronic urethroprostatitis. Recipe. 2016; 2: 169-73. [In Russian]

Rubanik L.V., Poleshchuk N.N., Skvortsova I.Y. Human papillomavirus infection and other pathogens (Chlamydia trachomatis, Herpes simplex virus, Trichomonas vaginalis) of the urogenital tract as the co-factors of inflammation and morphological transformation triggers. Medicinskaya panorama. 2016; 1: 17-22. [In Russian]

Rubanik L., Astachonok A., Poleshchuk N., Zhavnerko G. Chlamydia trachomatis detection using immunomagnet fluorescent nanoparticles. 8th Meeting of the European Society for Chlamydia Research, 6-9 Sept. 2016: A38. Available at: http://www.escr2016.co.uk/

Eysmont O.L., Pashkevich L.A., Mohammadi M.T., Murzich AE, Astashonok A.N., Rubanik L.V., Poleshchuk N.N Clinical and morphological characteristics and microbiological analyzes of the hip joint tissues in patients with avascular necrosis of the femoral head. Meditsinskie novosti. 2015; 12: 59-64. [In Russian]

Astashonok A., Poleschiuk N., Dokukina T., Rubanik L., Zhavnerko G., Machrov M., Pinchuk A., Korolevich P. Identification of neurodegeneration biomarkers in patients with cognitive impairments and detection protease resistant form of prion protein (PrP27–30) at different states of dementia. Psychiatry, psychotherapy and clinical psychology (Minsk). 2015; 2: 11-21. [In Russian]

Rubanik L.V., Astashonok A.N., Zhavnerko G.K., Polеshchuk N.N. Chlamydia trachomatis indication using immunomagnet fluorescent nanoparticles in case of persistent infection form. Healthcare. 2014; 10: 54-58. Available at: http://www.zdrav.by/indikaciya-chlamydia-trachomatis-s-pomoshchyu-immunomagnitnyh-flyuorescentnyh-nanochastic-pri или http://www.zdrav.by/pdf/2014/Zdrav10.pdf [In Russian]

Gavrusev A.A., Rubanik L.V., Strotsky A.V., Poleshchuk N.N. Peculiar clinical features of chronic urethroprostatitis after urogenital infections inefficient management. Healthcare. 2014; 2: 4-9. Available at: http://www.zdrav.by/osobennosti-kliniki-hronicheskogo-uretroprostatita-pri-neeffektivnom-lechenii-urogenitalnyh-infekciy или http://www.zdrav.by/pdf/2014/Zdrav2.pdf [In Russian]

Beletskiy A.V, Eismont O.L., Murzich A.E., Rubanik L.V., Paliashchuk N.N. Current status of diagnosis and treatment of avascular necrosis of the femoral head in adults. Meditsinskie novosti. 2013; 7: 25-30. Available at: http://cyberleninka.ru/article/n/sovremennoe-sostoyanie-problemy-diagnostiki-i-lecheniya-avaskulyarnogo-nekroza-golovki-bedra-u-vzroslyh [In Russian]

Poleschuyk N., Astashonok A., Rubanik L., Kapitulets S., Zhavnerko G., Paribok I., Farnia P., Yaminsky I. Nanotechnology approaches to the diagnostics of bacterial and viral infections. Nanoindustry (Moscow). 2012; 5: 48-55. Available at: http://www.nanoindustry.su/files/article_pdf/3/article_3358_815.pdf [In Russian]

Rubanik L.V., Poleshchuk N.N, Soroka N.F., Varonko I.a., Deikun D.A., Astashonok A.N., Kostyuk S.A., Poluyan O.S. Pheno- and genotypic resistance of Chlamydia trachomatis to antibiotics in patients with Chlamydiа induced arthropathies. Healthcare. 2011; 12: 51-55. Available at: http://www.zdrav.by/images/stories/2011-pdf/Zdrav12.pdf [In Russian]

Poleshchuk N.N., Rubanik L.V., Gavrusev A.A., Kapitulets N.N., Kostyuk S.A. Ultrastructural correlates of Trichomonas vaginalis Metronidazole resistance and protozoons transformation into various morphoforms under long-time culturing. Healthcare. 2010; 5: 29-33. [In Russian]

Khvorik D.F., Tsyrkunov V.M., Poleschuk N.N., Kostyuk S.A., Kapitulets N.N., Kapitulets S.P.,Rubanik L.V. Immunity formation under experimental chlamydial urogenital infection. Healthcare. 2008; 9: 61-64. [In Russian]

Skvortsova I.Yu., Peresada O.A., Rubanik L.V., Kostyuk S.A., Yagovdik N.Z., Poleshchuk N.N. Therapy of inflammatory processes caused by Trichomonas vaginalis combined with Chlamydia trachomatis in women with reproduction changes. Healthcare. 2006; 11: 50-54. [In Russian]

The Staff of the Laboratory,2014